Applications:

 Applications and Uses of Vectors:

  • Generation of gene library.
  • Development of Field Application Vectors for Bio-remediation of Soils.
  • yeast artificial chromosome libraries containing large inserts from mouse and human DNA
  • The applications of cloning vectors in recombinant DNA technology is immerse.
  • Bacterial Artificial Chromosome Library for Sequencing the Complete Human Genome
  • Constructing cDNA library 
  • Cloning DNA fragments
  • Generating Libraries: essential step for genome mapping
  • Positional cloning – discovering disease genes
  • Discovering genes from e.g. Protein sequence
  • Creating physical maps of large genome
  • Vectors that can yield the protein products of the cloned genes
  • It is used for making Vaccines
  • It is used in making hybrid plants

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Reference:

  • C. A. Lajoie, G. J. Zylstra, M. F. Deflaun AND P. F. Strom, Development of Field Application Vectors for Bioremediation of Soils Contaminated with Polychlorinated Biphenyls, American Society for Microbiology, Vol. 59, No. 6, p. 1735-1741
  • Z. Larin, P. M. ANTHONY AND L. Hans, Yeast artificial chromosome libraries containing large inserts from mouse and human DNA, PNAS, Vol. 88, pp. 4123-4127.
  • O . Kazutoyo , G. M. Aaron , W. Chenyan , F. Eirik, Z. Changjiang ,  C.J Joseph and J. Pieter, A Bacterial Artificial Chromosome Library for Sequencing the Complete Human Genome, Genome Research, Vol. 11:483–496
  • iGenetics - a molecular approch, Peter J. Russell, second edition, pearson benjamin cummings, 2006