Vectors for yeast and other fungi
Yeast integrative plasmids
Image source : Figure 7.3, Gene Cloning & DNA Analysis- An Introduction, T.A.Brown. fifth edition, blackwell publishing, 2010
Yeast integrative plasmids (YIps) are basically bacterial plasmids carrying a yeast gene.
YIp yields less than 1000 transformants per μg and has a single copy per cell.
An example is YIp5, which is pBR322 with an inserted URA3 gene. [4] This gene codes for orotidine-5′-phosphate decarboxylase and is used as a selectable marker. A YIp cannot replicate as a plasmid as it does not contain any parts of the 2 μm plasmid, and instead depends for its survival on integration into yeast chromosomal DNA. [4]
Reference:
- Principles and Techniques of Biochemistry and Molecular Biology , Wilson Keith and Walker John, seventh edition, cambridge university press, 2010
- Gene Cloning & DNA Analysis- An Introduction, T.A.Brown. fifth edition, blackwell publishing, 2010
